Isolation and screening of cellulosedegrading bacteria. Isolation is done to obtain pure bacterial cultures. In this way cellulose degrading bacteria were isolated from these six positive samples and bacteria were identified as two species of thermoactinomycetes sp. The cellulase producing bacteria were isolated from industrial and agricultural areas in kerala. The aim of this study is to isolate and characterize bacteria able to degrade starch from the rhizospheres of various plants at four. Bushnell haas medium bhm was used for isolation of cellulose hydrolytic bacteria after amendment with carboxymethylcellulose cmc as the sole carbon source.
A microscopic examination revealed that the isolated strain c 1 and c 2 was rod shaped and found to be gram positive where as c 3 strain was short rod in shape and found to be. The identification is required so as to cure the illness or the infection caused due to. Isolation and identification of cellulose degrading. Cellulose degrading bacteria were enriched and isolated by inoculating. Eight isolates of cellulosedegrading bacteria cdb were isolated from four different invertebrates termite, snail, caterpillar, and bookworm by enriching the basal culture medium with filter paper as substrate for cellulose degradation. This has been confirmed from the results of isolation of cellulosedegrading bacteria that produce clear zone beneath and around the colonies. The biochemical characterizations of the isolated strains c 1, c 2 and c 3 are presented in additional file 3. Pdf isolation and characterization of potential cellulose. This method was first described by hungate in 1947 and is still extensively used today, and widely regarded as the best method for the isolation of obligate anaerobic cellulosedegrading bacteria and fungi that cannot be cultivated on solid medium in petri dishes.
Biomass decomposition is considered to be the key process that supports all other biogeochemical processes. Isolation and screening of cellulose degrading bacteria in kitchen. Cellulase which produced by bacterial isolates are extracellular enzyme 1. Cellulolytic activity of cellulose degrading bacteria on cmc medium was assayed using degradation of whatmann no. Guts of the collected organism were separately crushed in 0. Jan 01, 2017 how to isolate cellulose degrading bacteria sajid shaikh. The isolates were identified based on morphology and biochemical characterization while the identities of the potent. Cellulose degrading bacteria were enriched and isolated by inoculating filter paper in liquid medium with macerated guts from termite, bookworm, snail, and caterpillar separately. Functiondriven singlecell genomics uncovers cellulose. Isolation and characterization of cellulolytic bacteria from. The isolates were identified based on morphology and biochemical characterization while the identities of the potent cellulolytic bacteria isolates were confirmed by 16srrna sequencing.
Isolation, screening and identification of cellulase producing bacteria. Pdf isolation and identification of cellulose degrading bacteria. Venkateswar reddy2 1centre for biotechnology, institute of science and technology, jawaharlal nehru technological university, hyderabad 500085, t. Cellulolytic microorganisms such as fungi and bacteria are responsible for much of the cellulose degradation in soils. India is the fifth largest producer of pineapple with an annual output of about 1. The macerated gut of the collected organisms was inoculated in a basal salt media nano 3 2. Studies on cellulose degrading microorganisms associated with rumen of ruminants was carried out from ruminants ram, cow, and goat, through culture, microscopic identification, biochemical test and cellulose degrading methods. Isolation and preliminary screening of lignin degrading microbes. Isolation and identification of cellulolytic bacteria from. Isolation of cellulose degrading bacteria from rumen and. Screening, purification and characterization of cellulase.
Starch degrading bacteria are important for different industries such as food, fermentation, textile, and paper. Isolation and characterization of cellulose degrading bacteria of termite gut from north eastern region of india special issue on. In the present study, cellulose degrading bacteria was isolated from sheep rumen. Essentially, the choice is between insoluble forms of cellulose, the degradation of which is a true indication of cellulolytic activity, or soluble derivatives such as carboxymethyl cellulose cmc, which is convenient to incorporate in isolation media, but can be degraded by many microorganisms that produce endoglucanases in the absence of significant activity against native cellulose. A total of 245 cellulose degrading aerobic bacterial strains were isolated from different natural reserves in the subtropical region of china, which were cultured in agar medium containing sugarcane bagasse pulp as the sole carbon source.
Isolation and screening of cellulose degrading bacteria in. All bacterial culture showed growth as the medium turned cloudy and the. Amylase activity of a starch degrading bacteria isolated. Screening isolation and characterization of cellulase. About one fifth of fresh water and soil samples yield cellulose degrading bacteria after enrichment but some samples did not bear such kind of bacteria 17. Isolation and characterization of starch degrading. Cellulose degrading bacteria can be used for commercial production of cellulase enzyme in which the enzyme is extracted by the use of bioprocessing technology from cellulolytic bacteria 1,2,3. Anand aap, vennison sj, sankar sg, probhu dig, vasan pt, raghuraman t, et al. All bacterial culture showed growth as the medium turned cloudy and the filter paper became macerated.
Isolation and screening of cellulose degrading microorganisms. Isolation, characterization and practical significance of cellulose degrading bacteria from the gut wall of two ecologically distinct earthworms kishore k. Isolation, screening and characterization of cellulolytic. International journal of applied microbiology science, 12. Isolation of cellulosedegrading bacteria and determination. Fortyone cellulose degrading bacteria cdb were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. Enrichment technique was used for isolating cdb cellulose degrading bacteria. Mesophilic aerobic gram negative cellulose degrading. A type of cellulase, endoglycosidase cleaves the disaccharide cellobiose from cellulose, and another type of enzyme. Isolation of cellulosedegrading bacteria and determination of. Isolation and characterization of cellulase producing. Cellulases are now used in the textile industry for cotton softening and denim finishing, in laundry.
Comparison of detachment procedures for direct counts of bacteria associated with sediment particles, plant litter and epiphytic biofilms. Isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. Aug, 2018 since cellulose is the most important component of plant biomass and a source of carbon, the cellulose degrading microorganisms cdms were isolated from these litter falls and screened for their ability to degrade carboxymethyl cellulose cmc in agar plates as substrate. Isolation and identification of cellulose degrading bacteria and optimization of the cellulase production joseph vimal, akhil venu, and jini joseph department of biotechnology, st. Isolation and identification of cellulose degrading bacteria. Fallow period of a jhum cycle is the recuperation phase of jhumming practice, where most of the processes like regeneration of secondary forest, equilibration of biomass forest litter fall decomposition and nutrient cycling processes take place. Cellulolytic organisms can also be used as probiotics in animal nutrition. Isolation, biochemical characterization, and phylogeny of. Pdf isolation and characterization of cellulolytic bacteria. Isolation and characterization of starch degrading rhizobacteria. Potential isolates with cellulase production were identified by. Pdf eight isolates of cellulosedegrading bacteria cdb were isolated from four different invertebrates termite, snail, caterpillar, and bookworm. Isolation and screening of cellulolytic bacteria from soil. This method was first described by hungate in 1947 and is still extensively used today, and widely regarded as the best method for the isolation of obligate anaerobic cellulose degrading bacteria and fungi that cannot be cultivated on solid medium in petri dishes.
Isolation, screening, and identification of cellulolytic. After 21 bacterial strains were isolated, the one with cellulose degrading ability were determined using enrichment technique. Isolated cellulose from corn cobs is used as the sole carbon source for cellulolytic bacteria which cultured in broth media. Request pdf isolation of cellulosedegrading bacteria the details of many microbial processes can only be clarified by using pure cultures. Isolation of cellulosedegrading bacteria request pdf. This study evaluates cellulose degrading bacteria isolates from paper mill dumpsites and their cellulolytic activity. Thatoi, isolation and identification of cellulose degrading bacteria from angrove msoil of mahanadi river delta and heir tcellulase production ability. Isolation and screening of cellulases producing bacteria. The number of cultivable bacteria per gut was calculated from the average number of colonies present on primary isolation plates pelczar et. This report is submitted in partial fulfillment of the requirements for the degree of bachelor of science with honours resource biotechnology faculty of resource science and technology university malaysia sara w ak 2006. Optimization and molecular identification of novel. Isolation and characterization of cellulosedegrading. How to isolate cellulose degrading bacteria sajid shaikh. Isolation of bacterial strains streaking plate method was used for obtaining pure bacterial strain.
Studies on cellulose degrading microorganisms associated with. These tissues are monitor organs that usually harbor the diseasecausing bacteria during infection. Methods for the isolation of cellulosedegrading microorganisms. Taken together, these data provided support for the efficacy of our approach and its utility for the targeted isolation of environmental bacteria that bind cellulose. Isolation and identification of cellulose degrading bacteria from.
Cellulose feeding organisms like termite, caterpillar, bookworm, and snail were collected for isolation of cellulosedegrading bacteria from woody habitats. Isolation of cellulose degrading bacteria from rumen and evaluation of cellulase production by the isolate using lignocellulosic substrate submission of manuscript for publication olyad erba urgessa haramaya university, college of natural and computational sciences, school of biological sciences and biotechnology. The steps of this study consisted of cellulose isolation, cellulolytic bacteria isolation and identification. Isolation and screening of cellulose degrading bacteria. This has been confirmed from the results of isolation of cellulose degrading bacteria that produce clear zone beneath and around the colonies. Cellulose degrading bacteria from termite cryptotermes sp.
Recent advances in biodegradation, sanitation, and bioremediation. Despite this vast number of cellulase producers, there is a deficiency of microorganisms that can produce significant amount of the three cellulase enzyme specifities i. Isolation and identification of cellulolytic bacteria from the gut of. Nuagada, atharabanki and mangrove forest at indian farmer. Among 12 isolates of bacteria, six isolates were cellulolytic. Isolation of cellulose degrading bacteria from rumen and evaluation of cellulase production by the isolate using lignocellulosic substrate. Isolation of cellulose degrading bacteria the soil samples were collected from different location of mangrove forest such as jumbo, kharnasi, triveni, 42 american journal of microbiological research. Isolation, screening and characterization of cellulolytic bacteria from forest soil sample korra ashwani1, lavudi saida1 and k. Bacteria are usually isolated from fish kidney and spleen. This is due to existence of microenvironments where different growth conditions for cellulose degrading bacteria are present. Gupta p, samant k, sahu a 2012 isolation of cellulosedegrading bacteria and determination of kadarmoidheen m, saranraj p, stella d 2012 effect of cellulolytic fungi on the degradation of cellulosic agricultural wastes. Therefore, the present investigation was designed to isolate and screen the cellulase producing bacteria from soil. Abstract this study was conducted to isolate cellulose degrading bacteria from mangrove soil of mahanadi river delta, odisha, india and also to evaluate their.
Isolation and characterization of potential cellulose. Isolation, screening and molecular identification of. Isolation and screening of cellulose degrading microbes. Isolation and screening of cellulose degrading microbes from. Results and discussion cellulose degrading bacteria were isolated from mangrove soils of mahanadi delta using cmc agar medium figure 1. Isolation and identification of cellulose degrading bacteria from different sources at assiut governorate upper egypt. Fortyone cellulosedegrading bacteria cdb were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. Cellulose degrading bacteria were isolated from mangrove soils of mahanadi delta using cmc agar medium figure 1. Pdf isolation and identification of cellulose degrading. How to isolate cellulose degrading bacteria youtube. Pdf isolation of cellulosedegrading bacteria and determination. Lignocellulose is the major structural component of woody plants and nonwoody plants such as grasses and represents a major source of renewable organic matter.
Peters college, kolenchery, kerala, india received june 02, 2016, accepted june 29, 2016 abstract. Isolation and preliminary screening of lignin degrading. Enrichment and isolation of mesophilic 37c and thermophilic 60c cellulosedegrading bacteria weathered soillike materials were used to isolate cellulose degrading mesophilic and thermophilic bacteria. Studies on cellulose degrading microorganisms associated. Isolationofcellulosedegradingbacteriaanddeterminationof. Ten grams of each soil or ward poultry sample were diluted with 90 ml saline solution in erlenmeyer flasks. Isolation of cellulolytic bacteria, including actinomycetes, from coffee exocarps in coffeeproducing areas in vietnam, international journal of recycling of organic waste in agriculture, 2014, pp. Isolation of cellulosedegrading bacteria springerlink. Isolation and characterization of potential cellulose degrading bacteria from sheep rumen article pdf available in journal of pure and applied microbiology 3.